Objective To evaluate the safety and efficacy of a new magnetic anchoring and guidance system on endoscopic submucosal dissection (ESD) in pig models. Methods Two skilled operators performed ESD at the rectum part of 2 pigs with the help of a new magnetic anchoring and guidance system. The dissection time, dissection rate per unit time, en-bloc resection rate and complications were analyzed．Results Five simulated lesions underwent ESD with the help of the magnetic anchoring and guidance system. The dissection time was 26.6 min and the dissection rate was 0.38±0.1 cm2/min. All the lesions were dissected totally. There was no perforation and delayed bleeding. Conclusion The new magnetic anchoring and guidance system is effective and safe for pig rectal ESD. It can effectively expose submucosal layer, and assist resection under the endoscopic observation.