Objective To investigate the value of endoscopic ultrasound （EUS）-guided drainage via upper gastrointestinal tract for peripancreatic fluid collection. Methods The clinical data of 103 patients with peripancreatic fluid collection undergoing EUS-guided drainage via upper gastrointestinal tract from May 2006 to July 2017 in Nanjing Drum Tower Hospital were retrospectively analyzed， the treatment and postoperative complications were summarized. Results Among the 103 patients， 101 （98.1％） were punctured successfully， and patients were punctured mostly through the gastric wall （97.1％， 100／103）. There were 29 cases of metal stent placement， and 66 plastic stent placement. Ten patients underwent ERCP with pancreatic stent placement. After operation， 8 patients underwent percutaneous drainage， and 1 surgical drainage. Complications occurred in 14 （13.6％） cases， including stent displacement in 5 cases， and postoperative infection in 9 cases. There was no perforation， bleeding， pancreatic fistula， other serious complications or death. Conclusion EUS-guided drainage is safe and reliable for peripancreatic fluid collection.