Objective To Study the clinical value of narrow-band imaging combined with magnifying endoscopy(ME-NBI) in biopsy of gastric mucosal low-grade intraepithelial neoplasia. Methods From January 2016 to June 2008, 148 patients with gastric mucosal low-grade intraepithelial neoplasia diagnosed by gastroscopy biopsy in the outpatient department of our hospital were included. All of them underwent ME-NBI, targeted biopsy pathology, ultrasound endoscopy, abdominal enhanced CT and ESD treatment, and the examination results and surgical conditions, postoperative pathology and complications of ESD were recorded in detail. Results ME-NBI examination, targeted biopsy under the guidance of ME-NBI, and postoperative pathological diagnosis of cancer lesions after ESD were respectively 19 cases, 17 cases, 20 cases, with no statistically significant difference between pairwise and pairwise (P > 0.05).Taking postoperative pathology of ESD as the final diagnosis result, the misdiagnosed rate of cancer lesions in conventional biopsy, ME-NBI examination and targeted biopsy under the guidance of ME-NBI were 13.51%, 0.68% and 2.03%, respectively. There were statistically significant differences in the misdiagnosed rate between conventional biopsy and ME-NBI examination and targeted biopsy (P < 0.05). There was no statistically significant difference in the misdiagnosed rate between ME-NBI examination and targeted biopsy (P > 0.05). Conclusion It is necessary to inspect ME-NBI for biopsy of gastric mucosal low-grade intraepithelial neoplasia. ME-NBI can improve the detection rate of early gastric cancer and reduce the misdiagnosed rate.