To investigate the correlation between different endoscopic ultrasonographic signs and pathological risk grade of gastric stromal tumors. Data of 89 patients with gastric stromal tumors confirmed by pathology and immunohistochemistry who underwent gastroscopy and endoscopic ultrasonography in Zhongnan Hospital of Wuhan University from March 2013 to December 2017 were retrospectively analyzed. The endoscopic ultrasonographic characteristics of gastric stromal tumors with different risk degrees were compared. Among the 89 cases, 63 were very low-risk grade, 21 were low-risk grade, and 5 were intermediate-risk grade. Tumor size, echo homogenicity, ulcer, with or without regular boundary, and cystic change were correlated with tumor risk grade (P<0.05). Endoscopic ultrasonography is helpful to predict risk grade of gastric stromal tumors before surgery.