Clinical data of 62 patients with gastrointestinal submucosal tumors (diameter ≥1 cm) who were treated with kissing suture by single‑channel endoscope after endoscopic full‑thickness resection (EFR) in the First Affiliated Hospital of Zhengzhou University from February 2017 to May 2019 were analyzed retrospectively to evaluate the clinical value of kissing suture technique by single‑channel endoscope in defect closure after EFR. All the post‑EFR defects were successfully closed (100%). No postoperative anastomotic leakage or other serious complications occurred. The mean maximum diameter of lesions was 3 cm (ranged 1‑7 cm), and 13 (21%) of them were greater than or equal to 5 cm.The mean number of clips used was 25.7 (ranged 7‑78). The mean procedure time was 168 min (ranged 44‑300 min), and the mean suture time was 63 min (ranged 13‑211 min). The mean postoperative hospital stay was 7 days (ranged 4‑12 days). Endoscopic reexamination 3‑6 months after operation showed that all wounds healed well. Kissing suture method by single‑channel endoscope is a safe, effective, easy and feasible for closing gastrointestinal defect after EFR.