The 13C urea breath test (13C-UBT) is the most commonly used non-invasive method for detecting Helicobacter pylori, but the quality control standards are uneven. Chinese Siociety of Digestive Diseases and Chinese Siociety of Digestive Endoscopology organized experts in related fields to provide suggestions on 13C-UBT management, quality control, testing procedures, result interpretation and other aspects, for reference of medical institutions where 13C-UBT are performed.