Objective To investigate the effect of air sterilization station on air quality control of digestive endoscopy center through a national multi-center dynamic monitoring research. Methods This study was conducted jointly with 15 digestive endoscopy centers in different regions of China. Each center selected 2 endoscopy rooms using level 4 and level 3 digestive endoscopy techniques, one with an air sterilization station (the experimental group) and the other without the device (the control group). The concentrations of PM5 and PM0.5 in the rooms before and after the use of air sterilization station were detected and compared. Results The air quality of all research units failed to reach the standard air cleanliness level 8 without using air sterilization station. After using the air sterilization station, the PM5 concentration and PM0.5 concentration of the experimental group in each center under dynamic conditions were lower than those of the control group, and the difference was significant (P<0.05). The PM5 concentration and PM0.5 concentration in the experimental group at each center showed a decreasing trend with start-up time, with significant difference at different start-up times (P<0.05). The PM5 concentration could reach the air cleanliness level 8 standard 15.7 minutes after the air sterilization station started, and the PM0.5 concentration could reach the air cleanliness level 8 standard 25.0 minutes after the air sterilization station started. Conclusion In the dynamic environment of the digestive endoscopy center, the air sterilization station can significantly improve the air quality in the endoscopy rooms, which is worth promoting in endoscopy rooms of medical institutions of all levels.